Optimize Your Trigger Mode Analysis with FlowCam 8400

Discover how to achieve precise and reliable results using Trigger mode on FlowCam 8400 and FlowCam Cyano instruments. This comprehensive technical note offers expert guidance on sample preparation, optimal dilution practices, and the mathematical models needed to ensure accurate data collection.

What You’ll Learn:

• Understanding PPUI (Particles Per Used Image): Why achieving a PPUI value of 1.20 or below is critical for accurate fluorescence measurements.
• Dilution Guidelines: Step-by-step instructions to quickly determine the ideal dilution factor for your samples.
• Mathematical Model: Use the provided equations to calculate dilution factors and reduce preparation time.
• Case Studies: Real-world examples showcasing the relationship between dilution ratios and PPUI values for various sample types.

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